PacBio Unveils CiFi Method for Chromosome-Scale Genome Assemblies in Single Sequencing Run

Reuters01-08
PacBio Unveils CiFi Method for Chromosome-Scale Genome Assemblies in Single Sequencing Run

Pacific Biosciences of California Inc. (PacBio) has announced the introduction of CiFi, a new long-read 3C (chromatin conformation capture) method developed in collaboration with researchers at the University of California, Davis. The results, which have already been published in Nature Communications, demonstrate that CiFi enables chromosome-scale, haplotype-resolved genome assemblies from a single sequencing run, even with limited sample material. By integrating 3C with PacBio’s HiFi long-read sequencing technology, CiFi produces highly accurate, multi-contact reads that improve mapping in repetitive genomic regions and simplify project workflows. In tests using prairie and meadow vole samples, the method delivered uncurated assemblies with scaffold N50 values exceeding 100 million base pairs and detected telomeric sequences at both ends of many scaffolds. The research highlights CiFi’s potential to lower costs and complexity for genome projects previously constrained by sample availability or budget.

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